ABSTRACT
Objective To investigate the effect of transforming growth factor β1 (TGF-β1) on the early reconstruction of Wistar rats rotator cuff tears through the tendon-bone healing biomechanical testing . Methods A total of 45 healthy Wistar rats were randomly divided into three groups: the high-dose, the low-dose, and the control group, with 15 animals in each group. The rats underwent full thickness leisure and reconstructive surgery by suture-bridge and TGF-β1 injection at the tendon bone interface. The parameters of tendon-bone interface biomechanics, including tensile strength, cross-section area, stiffness, percentage of loading, were measured at 1-, 2-, 3-weeks post surgery. Results No significant differences werey observed at the fracture point within the tendon suture among the three groups The maximum tensile strength , maximum load percentage, stiffness and tendon bone repair interface in the high-dose group were higher than those in the low-dose group and those in the control group(P < 0.05, respectively). The maximum axial loading, tensile strength and stiffness of the tendon-bone interface in the low-dose group were significantly higher than those in the control group (P < 0.05). The high-dose group renovated the tendon-bone interface healing and increase stiffness with the extension of the rehabilitation. Conclusion TGF-β1 can enhance the maximum tensile strength of the tendon-bone and promote the healing of rotator cuff injury.
ABSTRACT
This study was aimed to observe the effect of traditional Chinese medicine (TCM)Bu-Shen Gu-Jin Prescription (BSGJP) on the expression and action mechanism of interleukin-1 (IL-1) and tumour necrosis factor-alpha (TNF-α) in knee osteoarthritis (KOA) rabbit model. A total of 76 8-month healthy male New Zealand white rabbits were randomly divided into the control group, model group, TCM BSGJP group and western medicine group, with 19 rabbits in each group. Except the control group, improved Hulth modeling method was used in the animal model establishment. After 8-week consecutive medication, the serum, part of the synovial membrane tissues on the anterior side of the knee joint, articular cartilage and subchondral bone of the medial condyle of the femur were collected. Enzyme-linked immunosorbent assay (ELISA) was used in the content determination of IL-1 and TNF-α. The results showed that the narrowing of the joint space of the western medicine group were in between the model group and the TCM BSGJP group with obvious osteophyte formation. The TCM BSGJP group had slight narrowing of the joint space, mild rough of the articular cartilage surface, and slight osteophyte formation. The contents of IL-1 and TNF-α in the model group were higher than than of the control group (P < 0.01). Both medication groups were lower than the model group (P < 0.01). There was no significant difference between the two medication groups. It was concluded that BSGJP effectively reduced the secretion of IL-1 and TNF-α, delayed the deterioration of cartilage, which provided scientific experimental evidences for the clinical applications.
ABSTRACT
Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.